Intestinal epithelial SCs IESCs are

2018-10-20

Intestinal epithelial SCs (IESCs) are roughly categorized as either quiescent or active IESCs based in part on the expression of specific markers, including LGR5, Olfm4, Ascl2, BMI1, MTERT, and LRIG1 (Barker et al., 2007; Montgomery et al., 2011; Powell et al., 2012; Sangiorgi and Capecchi, 2008). T

tTA Tet Off exhibited high basal luciferase activity and mpC

2018-10-20

tTA2 (Tet-Off) exhibited high basal luciferase activity and mpCLOVER expression, similar to that of dox-induced rtTA-V10 (Figure 2D; Figure S2A4). mpCLOVER expression decreased with dox addition until it was undetectable at 5 days, and it rebounded 3 days after dox removal (Figures S2B4–S2D4). Lumin

We have developed a protocol for the

2018-10-20

We have developed a protocol for the efficient differentiation of functional HLCs from hPSCs that does not require the addition of recombinant growth factors and is applicable to both hESCs and hiPSCs. During the development of the protocol, it came as a surprise to discover that pluripotent Tasquin

Data generated from GSEA DAVID and gene hierarchical cluster

2018-10-20

Data generated from GSEA, DAVID and gene hierarchical clustering of sorted stem and committed progenitor cell transcriptomes provides an opportunity to explore processes that govern prostate stem cell biology, self-renewal and differentiation. One notable enrichment in active stem nucleoside analogs

It is interesting that OB

2018-10-20

It is interesting that OB neurons produced from the transient Gli1-enriched subcallosal domain in juvenile mice mostly corresponded to sGCs, when they were derived from a region with active SHH signaling. It is possible that the relative levels of SHH signaling are adjusted in different domains. The

Our study showed that neurite length and

2018-10-20

Our study showed that neurite length and the ratio of apoptotic compund w in FALS- and FUSH517D/H517D iPSC-derived neurons were the same as in control motor neurons. With several stresses especially in glutamate treatment, however, FALS- and FUSH517D/H517D HB9-positive motor neurons exhibited shorte

Among the epigenetic aberrations of iPSCs

2018-10-20

Among the epigenetic aberrations of iPSCs, DNA methylation is of particular importance. Previous studies showed that unique de novo differentially methylated (DMR) or hydroxymethylated regions (hDMR) are present in iPSCs compared with hESCs (Lister et al., 2011; Wang et al., 2013). Furthermore, the

Hello world!

2018-07-29